VA-ES-BJ細胞
年限： 41 years
數(shù)量： 大量
ATCC Number： CRL-2138?
相關**： 其他**
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
是否是腫瘤細胞： 1
物種來源： 人
Designations: VA-ES-BJ
VA-ES-BJ細胞Depositors: C Helson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Disease: epithelioid carcinoma
Derived from metastatic site: bone marrow
Cellular Products: granulocyte macrophage colony stimulating factor (GM-CSF)
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Tumorigenic: Yes
Oncogene:VA-ES-BJ細胞 p53 +
Cytogenetic Analysis: near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.
Age: 41 years
Gender: male
Ethnicity: Caucasian
Comments: VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an ***** male with primary vertebral epithelioid carcinoma.
The cells are sensitive to hemin.
The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.
VA-ES-BJ細胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22334: . . Int. J. VA-ES-BJ細胞Oncol. 7: 51-56, 1995.
