LL 86 (LeSa)細胞
器官來源： 肺
細胞形態(tài)： 成纖維樣
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 人
年限： 18 years
ATCC Number： CCL-190?
LL 86 (LeSa)細胞相關(guān)**： 正常
運輸方式： 凍存運輸
Designations: LL 86 (LeSa)
Depositors: RG Crystal
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
LL 86 (LeSa)細胞Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: normal human male; diploid
Isoenzymes: G6PD, B
Age: 18 years
LL 86 (LeSa)細胞Gender: male
Ethnicity: Caucasian
Comments: The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
LL 86 (LeSa)細胞Remove medium, rinse the monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
