1D3細胞
生長狀態： 懸浮生長
ATCC Number： HB-305?
細胞形態： **樣
**類型： IgG2a; kappa light chain
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 褐鼠
1D3細胞Designations: 1D3
Depositors: DT Fearon, I Krop, MS Schlissel, AR de Fougerolles
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 1D3細胞immunoglobulin; monoclonal antibody; against mouse CD19
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Comments: The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19.
The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19.
The antibody binds to mouse CD19 with high affinity, but does not react with human CD19.
It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19.
Propagation: 1D3細胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 34490: Krop I, et al. 1D3細胞Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073
