2A3A1H細胞
細胞形態： **樣
**類型： IgG1
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-8555?
2A3A1H細胞Designations: 2A3A1H
Depositors: Immunex Corporation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:2A3A1H細胞 immunoglobulin; monoclonal antibody; against human interleukin 2 (interleukin-2, IL-2) receptor
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with phytohemagglutinin activated human peripheral blood (PBL) leukocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody binds to IL-2 receptors on human leukocytes and blocks both mitogen induced and antigen induced PBL proliferation.
Propagation: 2A3A1H細胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 3994: March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989
3995: March CJ, et al. Interleukin-2 receptor. 2A3A1H細胞US Patent 4,578,335 dated Mar 25 1986
