gamma3-11.1細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數量： 大量
生長狀態： 懸浮生長
ATCC Number： HB-8700?
細胞形態： **樣
**類型： IgG1
gamma3-11.1細胞Designations: gamma3-11.1
Depositors: Takeda Chem. Ind., Ltd.
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: gamma3-11.1細胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human gamma interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
gamma3-11.1細胞Comments: Animals were immunized with a synthetic peptide (NH2-Lys-Arg-Lys-Arg- Ser-Gln-Met-Leu-Phe-Arg-Gly-Arg-Arg-Ala-Ser-Gln-COOH) conjugated to thyroglobulin.
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3909: Ichimori Y, et al. gamma3-11.1細胞Novel peptide and use thereof. US Patent 4,681,848 dated Jul 21 1987
