TIMI.4G.1.3細(xì)胞
細(xì)胞類型： T**細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
品系： C57BL/6
ATCC Number： TIB-38?
相關(guān)**： **瘤
TIMI.4G.1.3細(xì)胞生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: TIMI.4G.1.3
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6
Cell Type: TIMI.4G.1.3細(xì)胞T lymphocyte;
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Antigen Expression: H-2b; Thy-1.2
Comments: This line was derived from TIMI.4 (ATCC TIB-37).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: TIMI.4G.1.3細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1129: Hyman R. Studies on surface antigen variants. TIMI.4G.1.3細(xì)胞Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114
