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產品名稱: 主要堿性蛋白(MBP)單克隆抗體
產品型號: MBP
產品展商: HZbscience
產品文檔: 無相關文檔
簡單介紹
主要堿性蛋白(MBP)單克隆抗體是針對MBP的小鼠單克隆抗體。在**組織化學染色和**印跡實驗中能識別MBP。主要堿性蛋白(MBP)單克隆抗體主要用于WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM等多種**學實驗，一抗，二抗，標簽抗體，內參抗體、信號通路抗體、細胞凋亡抗體等現貨供應，獲得高靈敏度和高特異性的一致性結果的不二之選，產品靈敏度高，可重現性好，相同的名稱，不同的物種，多種規格供應,售后完善。
主要堿性蛋白(MBP)單克隆抗體
的詳細介紹
主要堿性蛋白(MBP)單克隆抗體
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來源單抗制備
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宿主小鼠
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效價(克隆號)1:270000 (2#)
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Ig亞型IgG1 Kappa
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純化方式蛋白A + 蛋白G親和純化
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主要堿性蛋白(MBP)單克隆抗體標記物無標記物
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度500ug/ml
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且適物種-
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應用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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主要堿性蛋白(MBP)單克隆抗體Monoclonal Antibody to Major Basic Protein (MBP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG1 Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: 2#
Traits: Liquid
Concentration: 500μg/mL
UOM: 200μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPB650Hu01-Recombinant Major Basic Protein (MBP)
主要堿性蛋白(MBP)單克隆抗體[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant MBP disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. 主要堿性蛋白(MBP)單克隆抗體[ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant MBP, Human
